Basic Molecular Biology: PCR and Real-Time PCR – Principle of PCR
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Amplifying DNA Polymerase Chain Reaction (PCR) is a biochemical technique that allows scientists to take tiny samples of DNA and amplify them into large samples that can then be examined to determine the DNA sequence. (This is useful, for example, in forensic science.) The process works by mixing the sample with appropriate enzymes and then heating it until the DNA double helix separates into two individual strands. The enzymes then copy each strand, and once the sample is cooled the number of D
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