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How to Thaw and Revive HEK 293 Cells: A Simple Guide
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YouTubeUcallm Biology
How to Thaw and Revive HEK 293 Cells: A Simple Guide
Thawing frozen HEK 293 cells properly is crucial for maintaining their viability and ensuring successful experiments. These adherent cells require careful handling during the thawing process to recover from cryopreservation. Here's a straightforward protocol to help you successfully revive your HEK 293 cells. Before You Begin: Preparation Warm ...
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Sharah Capinpin on Instagram: "Stop aggressively pipetting your cells into submission 🙅🏻‍♀️ After centrifugation, cells tend to pack TIGHTLY in a pellet = surface area exposed to buffer drops ⬇️. This prevents buffer (ie media, PBS, lysis buffers etc) to evenly come into contact with the cells. This can result in uneven mixing, poor recovery and inconsistent yield for downstream work like RNA or protein extraction. Plus, adding the buffer directly to a packed pellet can cause it to dislodge as
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