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How to Thaw and Revive HEK 293 Cells: A Simple Guide
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YouTubeUcallm Biology
How to Thaw and Revive HEK 293 Cells: A Simple Guide
Thawing frozen HEK 293 cells properly is crucial for maintaining their viability and ensuring successful experiments. These adherent cells require careful handling during the thawing process to recover from cryopreservation. Here's a straightforward protocol to help you successfully revive your HEK 293 cells. Before You Begin: Preparation Warm ...
13 views22 hours ago
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Sharah Capinpin on Instagram: "Stop aggressively pipetting your cells into submission 🙅🏻‍♀️ After centrifugation, cells tend to pack TIGHTLY in a pellet = surface area exposed to buffer drops ⬇️. This prevents buffer (ie media, PBS, lysis buffers etc) to evenly come into contact with the cells. This can result in uneven mixing, poor recovery and inconsistent yield for downstream work like RNA or protein extraction. Plus, adding the buffer directly to a packed pellet can cause it to dislodge as
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[RECOLTE 2025 💚] Place aux prochaines étapes pour transformer ces olives en un véritable jus de fruit 💛 Après le lavage, nos olives entrent dans les étapes clés de la transformation ! 👇 Broyage 🔨 Malaxage 👊 Séparation 💦 Centrifugation 🌪️ Chaque réglage compte pour préserver les arômes, la fraîcheur et la typicité de nos huiles ! 😋 Un savoir-faire collectif porté par nos mouliniers, fiers de transformer la récolte de nos adhérents en huile d'olive d'exception ✨ Encore un peu de patience..
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