Real-time PCR or quantitative PCR (Polymerase Chain Reaction) is an advancement of the standard PCR technique, which enables continuous monitoring of the reaction and collection of data in real time.

This is a preview. Log in through your library . Abstract Real-time polymerase chain reaction (real-time PCR), also known as quantitative PCR, is used to determine relative gene expression or to ...

With the increasing importance of generating large-scale data to drive new pharmaceutical and biotech discoveries, many scientists are feeling pressure to speed up data collection. One increasingly ...

Polymerase Chain Reaction (PCR) is a method which utilizes the mechanism of DNA replication to amplify a specific part of DNA. This involves the use of DNA polymerase, which makes new strands of DNA ...

Professors Michael Pfaffl (TU Munich, Weihenstephan, Germany) and Afif Nour (King Abdulaziz University, Saudi Arabia) designed the original MIQE qPCR app to help researchers improve their real-time ...

INTEGRA has reported how a leading US clinical diagnostic laboratory has, using a VIAFLO 96 handheld electronic pipette, significantly improved the reproducibility and productivity with which it ...

Real-time PCR plays a foundational role in gene expression studies, disease analysis, and molecular diagnostics. However, success depends on precise reaction setup, optimized assays, and accurate ...