In the MST experiment, R166A has a larger effect on hISG15 binding compared to Ub 2 or Ub 1 (Fig. 5f–h, green curve and Fig. 5i, green bars). Our calculations of interface energetics also indicated ...

We find the residue types compete closely at both sequence positions of the predicted patterns (Figure 3 a2,b2). The algorithm predicts an all-hydrophilic residue pattern, “PP”, as the best bitter ...