Researchers used a novel phosphate-activation reagent, 2-MeImIm-Cl, in an improved coupling reaction to increase the yield of ADP- or ATP-containing molecules. Unlike traditional reactions, the ...
In a recent study posted to the bioRxiv* preprint server, researchers discovered compounds able to inhibit severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) macrodomain 1 (Mac1) and ...
The circadian clock protein KaiC cyclically phosphorylates and dephosphorylates itself with a periodicity of ∼24 h. Unlike the reactions mediated by conventional protein phosphatases, ...
We constructed a fluorescent sensor of adenylate nucleotides by combining a circularly permuted variant of GFP with a bacterial regulatory protein, GlnK1, from Methanococcus jannaschii. The sensor's ...
ATP-ADP exchange was estimated in the presence of plasma membrane H+-ATPase of oat (Avena sativa) roots partially purified with Triton X-100 by measuring [14C]ATP formation from [14C]ADP. Most studies ...
Mitochondrial carriers are believed widely to be homodimers both in the inner membrane of the organelle and in detergents. The dimensions and molecular masses of the detergent and protein–detergent ...
An anion-sensitive ATP-dependent H+ transport in microsomal membranes from Zea mays L. coleoptiles was partially characterized using the pH gradient-dependent decrease of unprotonated neutral red. The ...
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