In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...

Such a thick layer can be observed when the liquid is held within a transparent container and the observer is looking at the top most portion of the liquid from the sides of the transparent container.

The body hairs of the Saharan silver ant cause total internal reflection of light to make the ants almost ten times more reflective, preventing overheating and yielding their silver sheen, according ...

Refraction close refractionProcess by which a wave changes speed and sometimes direction upon entering a denser or less dense medium, eg a light ray changes direction when refracted by a lens. from a ...

Science presenter Jon Chase explains total internal reflection. He demonstrates how total internal reflection can make a coin disappear inside a glass of water and bend a laser beam. The second ...

The Wolfson Bioimaging Facility provides total internal reflection fluorescence microscopy (TIRF) with its multi-laser, dual-camera Olympus/Abbelight SAFe360 system which also offers single molecule ...